Examined this effect on the mRNA encoding GADD34. They treated wild-type cells (S/S) with thapsigargin (TG) performed an immunoblot for GADD34, eIF-2, as well as phosphorylated eIF-2.

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Start from Manfredi Question C: Paradoxically, when phosphorylation of eIF2 phosphorylation inhibits global protein synthesis, there is a selective increase in translation of certain mRNAs. The best studied is that of GCN4.
Young et al. (2015, J Biol Chem 290, 28257)

examined this effect on the mRNA encoding GADD34. They treated wild-type cells (S/S) with thapsigargin (TG) performed an immunoblot for GADD34, eIF-2, as well as phosphorylated eIF-2.

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