PCR Lab report, Wolbachia, Mosquitos.

Words: 259
Pages: 1
Subject: Uncategorized

This semester, you and your colleagues taking Genetic Techniques used DNA barcoding to identify mosquitoes (or other organisms) and PCR screening to determine if those organisms harbored the Wolbachia endosymbiont. Think of yourselves as a research team all working together to put together datasets that can be used to address a single research objective. You will analyze and interpret the aggregate data generated from all members of the team, not just your personal results. The two datasets include the COI DNA sequences and a table of positive or negative screening results for Wolbachia.

Analyze this data using the relevant analytical techniques that you have learned in this course. These include measures of genetic distance (within and between species) and a phylogenetic tree. The raw presence/absence table for Wolbachia (the overall class results which I provide to you as a spreadsheet) must be summarized using an appropriate figure or table (do not copy/paste the spreadsheet into your report!). If you feel there are other analytical techniques that can be used to address your research objective, feel free to use them–but they must be relevant.

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