tep 1: All of the questions for the week are in a single Discussion post – you need to provide a short a succinct answer to each of my questions. Be brief and accurate using ~3-4 sentences for each answer, although longer is fine if needed. Number each of your answers to match my numbered questions; week 1 question 1 is 1.1, week 1 question 2 is 1.2, etc. You will not receive credit if you copy the answers directly from the textbook or from another student, including a shared internet resource – write the answers in your own words. Step 2: Choose one of my questions to write a follow-up question to and include your question at the bottom of this same post “My follow-up question to RLM question 1.1 is:…” Protein Purification Methods
Hemoglobin: Allostery and Evolution
Q5.1 – 2,3-BPG is a negative allosteric regulator of hemoglobin (Hb) oxygen binding. Explain how elevated levels of 2,3-BPG in red blood cells at high altitude (14,800 ft above sea level) lead to more efficient delivery of O2 to the tissues considering that less O2 binds to Hb in the lungs under these conditions.
Membrane Transport Proteins
Q5.2 – What explains the 10,000 to 1 selectivity of the potassium channel for K+ over Na+ considering that the atomic radii of these two ions differ by only 0.4 Angstroms.
The Actin-Myosin Motor
Q5.3 – What biochemical process must occur in the myosin head to initiate the “power stroke” in muscle contraction, which is characterized by a large protein conformational change? What conformational change is associated with ATP hydrolysis?
