Is Glu-97 directly involved in the reaction with the substrate (DHAP)? What other role could it be playing in the catalytic mechanism? Why might the mutation have such a profound effect on the catalytic activity of the enzyme?

answer each questions short to the point

1. Is Glu-97 directly involved in the reaction
with the substrate (DHAP)? What other role could it be playing in the catalytic
mechanism? Why might the mutation have such a profound effect on the catalytic
activity of the enzyme?

2. Why does the mutation of Glu-97 to aspartate (which maintains the same
functionality in the side chain) have such a large effect on the catalytic
activity?

PLease look at the attach files for more info!