BIOCHEMISTRY METHODOLOGY ON CREATING

Words: 275
Pages: 1
Subject: Uncategorized

The task is to outline the methodology for how to create novel oral polio vaccine for wildtype poliovirus 1. I will attach a link to a paper that created novel oral polio vaccine for type wildtype poliovirus 2, here is the paper name and link for guidance –> ‘ Engineering the Live-Attenuated Polio Vaccine to Prevent Reversion to Virulence ‘
Link –> https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(20)30230-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1931312820302304%3Fshowall%3Dtrue

TASK – outline the hypothesis
outline method on how to increase genetic stability of the sabin1 genome, and method on how to slow down the sabin1 virus to prevent reversion and lead to a safer vaccine
how to test that the new vaccine is safe and immunogenic – include the method
any statistical tests that would need to be conducted
Must be a novel study
Critically analyse any methods used to achieve the objectives whilst exploring strengths and shortcomings
Critically appraise the study
Spell out the appropriate expected outcome with reason
must include what types of experiment is being used example genome studies and specify the method being used

AIM –> To create a more genetically stable vaccine in order to aid the eradication of wild poliovirus type 1

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