In this discussion forum the learning objective is to identify the phenotype of T cells in the thymus and peripheral blood in health and disease. First, watch video #1 (23 min.) on T cell development (https://youtu.be/JeV-HuPq7CI ) and video #2 (7 min.) on T cell development (https://youtu.be/WQlF2UZO8KA ).
i) Question 1- In an individual with a genetic deficiency in MHC class I genes (i.e. no MHC class I expressed), which of the following T cell phenotypes would you expect to find in the thymus (C4-single positive, CD8-single positive, CD4 and CD8 double positive, CD4 and CD8 double negative)? Using complete sentences, justify your answer based on positive selection and/or negative selection, and provide evidence from the videos or readings to support your conclusions.
ii) Question 2 – In an individual with a genetic deficiency in MHC class I genes (i.e. no MHC class I expressed), which of the following T cell phenotypes would you expect to find in peripheral blood (C4-single positive, CD8-single positive, CD4 and CD8 double positive, CD4 and CD8 double negative)? Using complete sentences, justify your answer based on positive selection and/or negative selection, and provide evidence from the videos or readings to support your conclusions.
