It is a mauscriipt about how IkB alpha overexpression affect p65 translocation to the nucleus. I uploaded a file that contains all protocols we did in the lab that can be used to write the Methods section. Also, at the end of the file you will find an important research article about NF-KB signaling, and it is a great source to use and it has a similar method. I also attached the manuscriipt figure that you will be used to write a clear and concise figure legend. Please read the Manuscriipt Rubric and the file uploaded carefully and let me know if you need any clarifications. Thank you in advance.