1. Dynamic oxygen consumption rate: Use the data provided on excel spreadsheet and (a) calculate oxygen consumption rate (OCR) in mM/min for a cell culture at 24 Million viable cells/ml, and (b) calculate cell specific oxygen consumption rate in umol/cell/hr. Remember 100% air saturation means 0.2 mM of oxygen in solution.
2. Using the data (pH versus time) provided in Excel spreadsheet, calculate mixing time for the bioreactor at 10,000L scale at different agitation rates. Plot the mixing time against agitation rates (rpm). The bioreactor uses a pitched blade impeller.
3. Using the data (DO versus time) provided in Excel spreadsheet, calculate kla for oxygen at different agitation rates and at different gas flow rates. Make a plot of kla against agitation rate and another plot showing kla against gas flow rate. If you use Rate method in addition to integral method, you will get extra point.
